Partial Purification and Kinetics of Indoleacetic Acid Oxidase from Tobacco Roots
نویسندگان
چکیده
منابع مشابه
Promotion of indoleacetic Acid oxidase isoenzymes in tobacco callus cultures by indoleacetic Acid.
Indoleacetic acid oxidase in tobacco callus cultures (Nicotiana tabacum L., cv. White Gold) was composed of at least two groups of isoenzymes, which were distinctly different in electrophoretic mobilities and in responses to growth substances. Indoleacetic acid had dual effects; at low concentrations it promoted the development of two fast-migrating indoleacetic acid oxidase isoenzymes, but at ...
متن کاملPartial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
متن کاملPartial Purification and Characterisation of Polyphenol Oxidase from Tomatoes (Solanum Lycopersicum)
Polyphenol oxidase (PPO) from tomatoes was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. The activity of polyphenol oxidase was investigated in solanum lycopersicum. Spectrophotometric method was used to assay the enzyme activity and the kinetic constants - maximum enzyme velocity (Vmax) and Michealis - Menten constant (Km). Of the ...
متن کاملChanges in indoleacetic Acid oxidase isoenzymes in tobacco tissues after treatment with 2,4-dichlorophenoxyacetic Acid.
2,4-Dichlorophenoxyacetic acid had a multiple effect on the development of indoleacetic acid oxidase isoenzymes in tobacco callus tissues (Nicotiana tabacum, cv. White Gold) cultured in vitro, and the development of these isoenzymes was differentially associated with growth promotion or inhibition depending on the concentration of 2,4-dichlorophenoxyacetic acid. At low concentrations (0.1 to 1 ...
متن کاملpurification and partial characterization of barley oxalate oxidase
introduction: oxalate oxidase (ec.1, 2, 3, 4), an acute phase protein of plants, is used widely in agriculture, food industries, medical diagnosis of kidney stones and oxaluria. in this study oxalate oxidase from barley was purified and partially characterized. materials & methods: roots and sprouts of barley cultured in hydroponic states were separated and extracted. ion exchange resins of the...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Physiology
سال: 1966
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.41.7.1200